Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins
- 4 September 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 3 (9), 1527-1534
- https://doi.org/10.1038/nprot.2008.144
Abstract
The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2–3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.Keywords
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