Floral and insect-induced volatile formation in Arabidopsis lyrata ssp. petraea, a perennial, outcrossing relative of A. thaliana
Open Access
- 26 March 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Planta
- Vol. 230 (1), 1-11
- https://doi.org/10.1007/s00425-009-0921-7
Abstract
Volatile organic compounds have been reported to serve some important roles in plant communication with other organisms, but little is known about the biological functions of most of these substances. To gain insight into this problem, we have compared differences in floral and vegetative volatiles between two closely related plant species with different life histories. The self-pollinating annual, Arabidopsis thaliana, and its relative, the outcrossing perennial, Arabidopsis lyrata, have markedly divergent life cycles and breeding systems. We show that these differences are in part reflected in the formation of distinct volatile mixtures in flowers and foliage. Volatiles emitted from flowers of a German A. lyrata ssp. petraea population are dominated by benzenoid compounds in contrast to the previously described sesquiterpene-dominated emissions of A. thaliana flowers. Flowers of A. lyrata ssp. petraea release benzenoid volatiles in a diurnal rhythm with highest emission rates at midday coinciding with observed visitations of pollinating insects. Insect feeding on leaves of A. lyrata ssp. petraea causes a variable release of the volatiles methyl salicylate, C11- and C16-homoterpenes, nerolidol, plus the sesquiterpene (E)-β-caryophyllene, which in A. thaliana is emitted exclusively from flowers. An insect-induced gene (AlCarS) with high sequence similarity to the florally expressed (E)-β-caryophyllene synthase (AtTPS21) from A. thaliana was identified from individuals of a German A. lyrata ssp. petraea population. Recombinant AlCarS converts the sesquiterpene precursor, farnesyl diphosphate, into (E)-β-caryophyllene with α-humulene and α-copaene as minor products indicating its close functional relationship to the A. thaliana AtTPS21. Differential regulation of these genes in flowers and foliage is consistent with the different functions of volatiles in the two Arabidopsis species.Keywords
This publication has 55 references indexed in Scilit:
- Identification and Regulation of TPS04/GES, anArabidopsisGeranyllinalool Synthase Catalyzing the First Step in the Formation of the Insect-Induced Volatile C16-Homoterpene TMTTPlant Cell, 2008
- A Maize (E)-β-Caryophyllene Synthase Implicated in Indirect Defense Responses against Herbivores Is Not Expressed in Most American Maize VarietiesPlant Cell, 2008
- Poorly known relatives of Arabidopsis thalianaTrends in Plant Science, 2006
- Diversity and Distribution of Floral ScentThe Botanical Review, 2006
- Recruitment of entomopathogenic nematodes by insect-damaged maize rootsNature, 2005
- Inducible indirect defence of plants: from mechanisms to ecological functionsBasic and Applied Ecology, 2003
- Sensitivity and specificity of atmospheric trace gas detection by proton-transfer-reaction mass spectrometryInternational Journal of Mass Spectrometry, 2003
- Defensive Function of Herbivore-Induced Plant Volatile Emissions in NatureScience, 2001
- Variability in herbivore-induced odour emissions among maize cultivars and their wild ancestors (teosinte)Chemoecology, 2001
- On-line monitoring of volatile organic compounds at pptv levels by means of proton-transfer-reaction mass spectrometry (PTR-MS) medical applications, food control and environmental researchInternational Journal of Mass Spectrometry and Ion Processes, 1998