Specific Endocrine Tissue Marker Defined by a Monoclonal Antibody
- 11 November 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 222 (4624), 628-630
- https://doi.org/10.1126/science.6635661
Abstract
One of two mouse monoclonal antibodies (LK2H10) produced by hybridoma technology against a human endocrine tumor (pheochromocytoma) demonstrated specific immunoreactivity for 69 normal and neoplastic endocrine and tissues known to contain secretory granules. This immunoreactivity was specific, since other normal tissues, tumors from endocrine cells without granules, and tumors from other nonendocrine tissues were negative when tested with antibody LK2H10. The antibody reacted with human fetal adrenal medulla and human pancreatic endocrine cells and with adrenal medullary cells from monkeys and pigs. The antigen detected by antibody LK2H10 is associated with cytoplasmic secretory granules, has an estimated molecular weight of 68,000, and may be related to human chromogranin.This publication has 11 references indexed in Scilit:
- Virus-Induced Autoimmunity: Monoclonal Antibodies That React with Endocrine TissuesScience, 1983
- Contributions of Immunohistochemistry to Clinical Endocrinology and Endocrine PathologyJournal of Histochemistry & Cytochemistry, 1983
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Neurone-specific enolase is a molecular marker for peripheral and central neuroendocrine cellsNature, 1978
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- The Neural Crest and the Origin of the Insulin-Producing and Other Gastrointestinal Hormone-Producing CellsScience, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- THE CYTOCHEMISTRY AND ULTRASTRUCTURE OF POLYPEPTIDE HORMONE-PRODUCING CELLS OF THE APUD SERIES AND THE EMBRYOLOGIC, PHYSIOLOGIC AND PATHOLOGIC IMPLICATIONS OF THE CONCEPTJournal of Histochemistry & Cytochemistry, 1969