PRODUCTION OF TOXIN IN VITRO BY BACILLUS ANTHRACIS AND ITS SEPARATION INTO TWO COMPONENTS
- 1 March 1960
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 79 (3), 450-455
- https://doi.org/10.1128/jb.79.3.450-455.1960
Abstract
B. anthracis produced toxin in a casamino acids medium without added serum or other protein. The toxin was separated into 2 components by filtering through fritted glass filters. One component, which appears to be the protective antigen, was present in the filtrate. The other component, referred to as filter factor, was adsorbed on the glass filter and could be eluted with alkaline buffer. Each of the factors was nontoxic when tested alone but when added together they formed a toxic mixture as evidenced by skin reactions in guinea pigs and lethality tests in mice. The toxin was neutralized by B. anthracis antiserum.Keywords
This publication has 6 references indexed in Scilit:
- Production in vitro of the Toxin of Bacillus anthracis Previously Recognized in vivoJournal of General Microbiology, 1958
- FURTHER PURIFICATION STUDIES ON THE PROTECTIVE ANTIGEN OFBACILLUS ANTHRACISPRODUCEDIN VITROJournal of Bacteriology, 1958
- An Agar-Diffusion Method for Titrating Bacillus anthracis Immunizing Antigen and its Application to a Study of Antigen ProductionJournal of General Microbiology, 1957
- The chemical basis of the virulence of Bacillus anthracis. VII. Two components of the anthrax toxin: their relationship to known immunising aggressins.1956
- THE CHEMICAL BASIS OF THE VIRULENCE OF BACILLUS-ANTHRACIS .5. THE SPECIFIC TOXIN PRODUCED BY B-ANTHRACIS INVIVO1955
- Studies on a protective antigen produced in vitro from Bacillus anthracis: medium and methods of production.1954