Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation
Open Access
- 9 March 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 31 (8), 1975-1984
- https://doi.org/10.1038/emboj.2012.56
Abstract
Eukaryotic mRNA decapping proteins are essential for normal turnover of mRNA. Yet, the mechanism of bulk mRNA turnover during stress responses and its importance to stress tolerance are poorly understood. Here, we showed that dehydration stress activated MPK6 to phosphorylate serine 237 of Arabidopsis DCP1 and phospho‐DCP1 preferentially associated with DCP5 to promote mRNA decapping in vivo . This process was essential for stress adaption as dcp5‐1 and DCP1‐S237A plants were hypersensitive to stress compared with wild‐type (WT) plants. Microarray analysis revealed that dehydration‐induced expression of many stress responsive genes was compromised in dcp5‐1 , whereas a subset of transcripts was over‐represented in this mutant. Further analysis revealed that this subset of transcripts was likely the direct targets of stress‐triggered mRNA decapping in WT. Our results suggest that mRNA decapping through MPK6‐DCP1–DCP5 pathway serves as a rapid response to dehydration stress in Arabidopsis.Keywords
This publication has 32 references indexed in Scilit:
- Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiaeThe Journal of cell biology, 2010
- DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoaProceedings of the National Academy of Sciences, 2009
- Arabidopsis Decapping 5 Is Required for mRNA Decapping, P-Body Formation, and Translational Repression during Postembryonic DevelopmentPlant Cell, 2009
- Similar Modes of Interaction Enable Trailer Hitch and EDC3 To Associate with DCP1 and Me31B in Distinct Protein ComplexesMolecular and Cellular Biology, 2008
- Arabidopsis MAPKs: a complex signalling network involved in multiple biological processesBiochemical Journal, 2008
- Structural Basis of Dcp2 Recognition and Activation by Dcp1Molecular Cell, 2008
- A Divergent Sm Fold in EDC3 Proteins Mediates DCP1 Binding and P-Body TargetingMolecular and Cellular Biology, 2007
- Components of theArabidopsismRNA Decapping Complex Are Required for Early Seedling DevelopmentPlant Cell, 2007
- The Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisPlant Cell, 2007
- ArabidopsisDCP2, DCP1, and VARICOSE Form a Decapping Complex Required for Postembryonic DevelopmentPlant Cell, 2006