Physiological regulation of a decontrolled lac operon
- 31 March 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 130 (1), 212-222
- https://doi.org/10.1128/jb.130.1.212-222.1977
Abstract
The expression of the lac operon was studied under a variety of growth conditions in induced and in constitutive cells of Escherichia coli that carried different catabolite-insensitive lac promoters. Use of such decontrolled lac operons permitted a study of the expression of an operon that was presumably subject only to passive control. Since the use of toluenized cells was demonstrated not to be completely reliable, all enzyme assays were performed on sonic supernatant fluids. The cells contained different catabolite-insensitive promoters, which included the L1 and UV5 lac promoters and others isolated in this study. Small but real C source effects were seen. There was only a small change in .beta.-galactosidase specific activity with changes in the growth rate, implying limited transcription and/or translation capacity within the cell. At rapid growth rates most promoters exhibited a decreased expression. The UV5 promoter, which was the strongest promoter, was an exception. A mechanism to explain this promoter-dependent control is discussed.This publication has 28 references indexed in Scilit:
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