Molecular cloning of cDNA for vitamin D3 25‐hydroxylase from rat liver mitochondria
- 12 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (1), 135-138
- https://doi.org/10.1016/0014-5793(90)80172-f
Abstract
A cDNA clone encoding mitochondrial vitamin D3 25-hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 1.9 kbp long and contained a 1599 bp open reading frame encoding 533 amino acid residues. The deduced primary structure contained a presequence typical for mitochondrial enzymes in the N-terminal region. The N-terminal sequence of the mature enzyme was determined to be Ala-Ile-Pro-Ala-Ala, which agrees perfectly with a portion of the deduced sequence, establishing the cleavage point of the precursor.Keywords
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