Cudraflavanone B Isolated from the Root Bark of Cudrania tricuspidata Alleviates Lipopolysaccharide-Induced Inflammatory Responses by Downregulating NF-κB and ERK MAPK Signaling Pathways in RAW264.7 Macrophages and BV2 Microglia
- 6 August 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Inflammation
- Vol. 44 (1), 104-115
- https://doi.org/10.1007/s10753-020-01312-y
Abstract
A prenylated flavonoid, cudraflavanone B, is isolated from Cudrania tricuspidata. In this study, we investigated its anti-inflammatory and anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced RAW264.7 and BV2 cells. In our initial study of the anti-inflammatory effects of cudraflavanone B the production of nitric oxide and prostaglandin E2 was attenuated in LPS-stimulated RAW264.7 and BV2 cells. These inhibitory effects were related to the downregulation of inducible nitric oxide synthase and cyclooxygenase-2. In addition, cudraflavanone B suppressed the production of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α in LPS-induced RAW264.7 and BV2 cells. Moreover, the evaluation of the molecular mechanisms underlying the anti-inflammatory effects of cudraflavanone B revealed that the compound attenuated the nuclear factor-kappa B signaling pathway in LPS-induced RAW264.7 and BV2 cells. In addition, cudraflavanone B inhibited the phosphorylation of extracellular signal-regulated kinase mitogen-activated protein kinase signaling pathways in these LPS-stimulated cells. Thus, cudraflavanone B suppressed nuclear factor-κB, and extracellular signal-regulated kinase mitogen-activated protein kinase mediated inflammatory pathways, demonstrating its potential in the treatment of neuroinflammatory conditions.Keywords
Funding Information
- The current study was supported by a research fund from Chosun University in 2016 (K207334002)
This publication has 53 references indexed in Scilit:
- Nitric oxide synthases: regulation and functionEuropean Heart Journal, 2011
- The pro- and anti-inflammatory properties of the cytokine interleukin-6Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2011
- Anti-inflammatory Effect of Glycoprotein Isolated fromCudrania TricuspidataBureau: Involvement of MAPK/NF-κB SignalingImmunological Investigations, 2010
- Ethyl Acetate Fraction from Cudrania Tricuspidata Inhibits IL-1β-Stimulated Osteoclast Differentiation through Downregulation of MAPKs, c-Fos and NFATc1The Korean Journal of Internal Medicine, 2010
- Macrophage interleukin‐6 and tumour necrosis factor‐α are induced by coronavirus fixation to Toll‐like receptor 2/heparan sulphate receptors but not carcinoembryonic cell adhesion antigen 1aImmunology, 2009
- Emerging roles of PGE2 receptors in models of neurological diseaseProstaglandins & Other Lipid Mediators, 2009
- Anti-inflammatory action ofCudrania tricuspidataon spleen cell and T lymphocyte proliferationJournal of Pharmacy and Pharmacology, 2008
- MAPKs and their relevance to arthritis and inflammationRheumatology, 2007
- Cudraflavanone A purified from Cudrania tricuspidata induces apoptotic cell death of human leukemia U937 cells, at least in part, through the inhibition of DNA topoisomerase I and protein kinase C activityAnti-Cancer Drugs, 2007
- Activators and target genes of Rel/NF-κB transcription factorsOncogene, 1999