Alternaria sp. MG1, a resveratrol-producing fungus: isolation, identification, and optimal cultivation conditions for resveratrol production
- 13 April 2012
- journal article
- biotechnological products-and-process-engineering
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 95 (2), 369-379
- https://doi.org/10.1007/s00253-012-4045-9
Abstract
Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4′-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol production. Endophytes from Merlot wine grapes (Vitis vinifera L. cv. Merlot), wild Vitis (Vitis quinquangularis Rehd.), and Japanese knotweed (Polygonum cuspidatum Siebold & Zucc.) were isolated, and their abilities to produce resveratrol were evaluated. A total of 65 isolates were obtained and 21 produced resveratrol (6–123 μg/L) in liquid culture. The resveratrol-producing isolates belonged to seven genera, Botryosphaeria, Penicillium, Cephalosporium, Aspergillus, Geotrichum, Mucor, and Alternaria. The resveratrol-producing capability decreased or was completely lost in most isolates after three rounds of subculture. It was found that only the strain Alternaria sp. MG1 (isolated from cob of Merlot using GA1 medium) had stable and high resveratrol-producing capability in all subcultures. During liquid cultivation of Alternaria sp. MG1 in potato dextrose medium, the synthesis of resveratrol began on the first day, increased to peak levels on day 7, and then decreased sharply thereafter. Cell growth increased during cultivation and reached a stable and high level of biomass after 5 days. The best fermentation conditions for resveratrol production in liquid cultures of Alternaria sp. MG1 were an inoculum size of 6 %, a medium volume of 125 mL in a 250-mL flask, a rotation speed of 101 rpm, and a temperature of 27 °C.Keywords
This publication has 58 references indexed in Scilit:
- Fungal endophytes: unique plant inhabitants with great promisesApplied Microbiology and Biotechnology, 2011
- Extraction of resveratrol from the pomace of Palomino fino grapes by supercritical carbon dioxideJournal of Food Engineering, 2010
- Metabolites from the endophytic mitosporic Dothideomycete sp. LRUB20Phytochemistry, 2009
- Metabolic engineering for plant natural product biosynthesis in microbesCurrent Opinion in Biotechnology, 2008
- Enriched ozone atmosphere enhances bioactive phenolics in seedless table grapes after prolonged shelf lifeJournal of the Science of Food and Agriculture, 2007
- Production of Resveratrol in Recombinant MicroorganismsApplied and Environmental Microbiology, 2006
- Therapeutic potential of resveratrol: the in vivo evidenceNature Reviews Drug Discovery, 2006
- Bioreactor studies on the endophytic fungusEntrophospora infrequensfor the production of an anticancer alkaloid camptothecinCanadian Journal of Microbiology, 2006
- Molecular biological studies on the pathogenicity of Alternaria alternata tomato pathotypeJournal of General Plant Pathology, 2004
- Fungal endophytes limit pathogen damage in a tropical treeProceedings of the National Academy of Sciences, 2003