Pleiotropic Roles of Polyglycerolphosphate Synthase of Lipoteichoic Acid in Growth of Staphylococcus aureus Cells
Open Access
- 1 January 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 191 (1), 141-151
- https://doi.org/10.1128/jb.01221-08
Abstract
Lipoteichoic acid (LTA) is one of two anionic polymers on the surface of the gram-positive bacterium Staphylococcus aureus . LTA is critical for the bacterium-host cell interaction and has recently been shown to be required for cell growth and division. To determine additional biological roles of LTA, we found it necessary to identify permissive conditions for the growth of an LTA-deficient mutant. We found that an LTA-deficient S. aureus Δ ltaS mutant could grow at 30°C but not at 37°C. Even at the permissive temperature, Δ ltaS mutant cells had aberrant cell division and separation, decreased autolysis, and reduced levels of peptidoglycan hydrolases. Upshift of Δ ltaS mutant cells to a nonpermissive temperature caused an inability to exclude Sytox green dye. A high-osmolarity growth medium remarkably rescued the colony-forming ability of the Δ ltaS mutant at 37°C, indicating that LTA synthesis is required for growth under low-osmolarity conditions. In addition, the Δ ltaS mutation was found to be synthetically lethal with the Δ tagO mutation, which disrupts the synthesis of the other anionic polymer, wall teichoic acid (WTA), at 30°C, suggesting that LTA and WTA compensate for one another in an essential function.Keywords
This publication has 52 references indexed in Scilit:
- dnaB and dnaI temperature-sensitive mutants of Staphylococcus aureus: evidence for involvement of DnaB and DnaI in synchrony regulation of chromosome replicationMicrobiology, 2007
- A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface properties and autolysin activityMolecular Microbiology, 2007
- A Metabolic Sensor Governing Cell Size in BacteriaCell, 2007
- Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureusProceedings of the National Academy of Sciences of the United States of America, 2007
- Colony Spreading in Staphylococcus aureusJournal of Bacteriology, 2007
- Wall Teichoic Acid Polymers Are Dispensable for Cell Viability in Bacillus subtilisJournal of Bacteriology, 2006
- Comparison of Lipoteichoic Acid from Different Serotypes of Streptococcus pneumoniaePublished by Elsevier BV ,2006
- Lesions in Teichoic Acid Biosynthesis in Staphylococcus aureus Lead to a Lethal Gain of Function in the Otherwise Dispensable PathwayJournal of Bacteriology, 2006
- Genetic identification of two distinct DNA polymerases, DnaE and PolC, that are essential for chromosomal DNA replication in Staphylococcus aureusMolecular Genetics and Genomics, 2001
- Phosphatidyl glycerolphosphate serves as glycerolphosphate donor in polymer synthesisBiochemical and Biophysical Research Communications, 1985