Separation of Lipase and Esterase from Rat Epididymal Adipose Tissue

Abstract
Rat epididymal adipose tissue contains a lipase [EC 3.1.1.1]. Esters of long chain fatty acids are preferentially hydrolyzed by the lipase. On the other hand, esters of short chain fatty acids are preferentially hydrolyzed by the esterase. Both alcohol moiety of esters and position of glycerides are not factors to differentiate between the lipase and the esterase. Purification of these lipase and esterase is also reported.