INTRACELLULAR CHANGES DUE TO NEUTRAL RED AS REVEALED IN THE PANCREAS AND KIDNEY OF THE MOUSE BY THE ELECTRON MICROSCOPE
Open Access
- 1 February 1955
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 101 (2), 213-224
- https://doi.org/10.1084/jem.101.2.213
Abstract
The effects of sublethal amounts of the cationic dye, neutral red, upon the structure of pancreatic exocrine cells, and upon the mitochondria of renal distal tubule cells, have been studied with the electron microscope. It was found that neutral red is a cytoplasmic toxin which causes reproducible and characteristic changes in the ergastoplasm, the zymogen granules, the mitochondria, and possibly in the Golgi complex. Ergastoplasmic membranes and granules and zymogen granules lose definition and become continuous with the cytoplasmic matrix. Mitochondria lose their internal folds, develop vacuoles which contain a solution of neutral red in high concentration, and form the nidus for the development of sudanophilic, argyrophilic, osmiophilic inclusions which appear in the cytoplasm after neutral red administration. Golgi granules, one of the three elements of the Golgi complex, appear to increase in number and to be scattered more widely through the cytoplasm than is normal. No consistent changes were found in the cell membrane or nucleus.Keywords
This publication has 5 references indexed in Scilit:
- Cytological Staining Procedures Applicable to Methacrylate-Embedded TissuesStain Technology, 1954
- Age Changes in the Fine Structure of Anterior Pituitary of the Mouse.Experimental Biology and Medicine, 1953
- Studies on the golgi substance of the epithelial cells of the epididymis and duodenum of the mouseJournal of Anatomy, 1953
- Improved Knife-Holders for Thin-sectioning with Rotary Microtomes.Experimental Biology and Medicine, 1953
- A STUDY OF FIXATION FOR ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1952