Cloning and sequence analysis of the human parathyroid hormone gene region
- 1 January 1990
- journal article
- research article
- Published by Springer Science and Business Media LLC in Human Genetics
- Vol. 84 (2), 119-124
- https://doi.org/10.1007/bf00208924
Abstract
A region of 50 kb around the human PTH gene was cloned and mapped by restriction analysis. Sequence analysis was performed and 3270bp determined, completing the sequence of the gene. The nucleotide sequence was analysed with regard to homology between human, bovine and rat PTH genes, and various potential cis-acting regulatory elements were identified. The gene region lacks an obvious CpG island. The PTH gene region in patients suffering from (pseudo)-hypoparathyroidism was investigated by Southern blotting. No detectable alteration in the fragment patterns was observed. Results of segregation analysis in families with affected individuals was inconclusive.Keywords
This publication has 33 references indexed in Scilit:
- The loci for parathyroid hormone and ?-globin are closely linked and map to chromosome 15 in cattleGenomics, 1988
- CpG Islands in vertebrate genomesJournal of Molecular Biology, 1987
- Deletion of lysine 13 alters the structure and function of parathyroid hormoneMolecular and Cellular Endocrinology, 1987
- Exclusion of close linkage between the parathyroid hormone gene and a mutant gene locus causing idiopathic hypoparathyroidism.Journal of Medical Genetics, 1986
- CpG-rich islands and the function of DNA methylationNature, 1986
- Molecular Biology of Parathyroid HormonCritical Reviews in Biochemistry, 1986
- Chromosome mapping of genes on the short arm of human chromosome 11: parathyroid hormone gene is at 11p15 together with the genes for insulin, c-Harvey-ras 1, and β-hemoglobinCytogenetic and Genome Research, 1985
- A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNACell, 1985
- Isolation of a functional human interleukin 2 gene from a cosmid library by recombination in vivoGene, 1984
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980