Cytoplasmic structure and contractility: the solation-contraction coupling hypothesis
- 4 November 1982
- journal article
- research article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. B, Biological Sciences
- Vol. 299 (1095), 185-197
- https://doi.org/10.1098/rstb.1982.0125
Abstract
We have briefly described our studies of cytoskeletal and contractile elements in intact cells, in cell extracts, and in mixtures of purified proteins. Changes in the concentration of calcium and of protons have been found to modulate both gelation and contraction in all of these preparations. The distribution of calcium, protons and actin has been studied in intact amoeboid cells. Using these results, we have refined our working model of the relation of cytoskeletal and contractile proteins: the solation-contraction coupling hypothesis. The model is also supported by quantitative analysis of the rates of contraction in a soluble extract of Dictyostelium discoideum amoebae allowed to gel in a capillary and stimulated by the addition of calcium ions at one end. A plausible interpretation of the most prominent cytological features of amoeboid locomotion is obtained by application of the principles of our model. In addition, we propose that the solation-contraction coupling hypothesis may be useful in further study of a variety of motile phenomena observed in many types of cells.This publication has 35 references indexed in Scilit:
- A 40,000-dalton protein from Dictyostelium discoideum affects assembly properties of actin in a Ca2+-dependent manner.The Journal of cell biology, 1982
- A calcium‐ and pH‐regulated actin binding protein from D. discoideumCell Motility, 1982
- Relation between cell activity and the distribution of cytoplasmic actin and myosin.The Journal of cell biology, 1981
- A new protein that gels F actin in the cell cortex of Dictyostelium discoideumNature, 1981
- Intracellular pH in single motile cells.The Journal of cell biology, 1980
- The contractile basis of ameboid movement. VI. The solation-contraction coupling hypothesis.The Journal of cell biology, 1979
- APPLICATIONS OF CALCIUM‐SENSITIVE PHOTOPROTEINS IN EXPERIMENTAL BIOLOGYPhotochemistry and Photobiology, 1978
- The contractile basis of amoeboid movement: V. The control of gelation, solation, and contraction in extracts from dictyostelium discoideumThe Journal of cell biology, 1977
- Mechanical properties of sea urchin eggsExperimental Cell Research, 1963
- Streaming in Cytoplasm Dissociated from the Giant Amœba, Chaos ChaosNature, 1960