Central Action of Peripherally Applied Botulinum Toxin Type A on Pain and Dural Protein Extravasation in Rat Model of Trigeminal Neuropathy
Open Access
- 4 January 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (1), e29803
- https://doi.org/10.1371/journal.pone.0029803
Abstract
Infraorbital nerve constriction (IoNC) is an experimental model of trigeminal neuropathy. We investigated if IoNC is accompanied by dural extravasation and if botulinum toxin type A (BoNT/A) can reduce pain and dural extravasation in this model. Rats which developed mechanical allodynia 14 days after the IoNC were injected with BoNT/A (3.5 U/kg) into vibrissal pad. Allodynia was tested by von Frey filaments and dural extravasation was measured as colorimetric absorbance of Evans blue - plasma protein complexes. Presence of dural extravasation was also examined in orofacial formalin-induced pain. Unilateral IoNC, as well as formalin injection, produced bilateral dural extravasation. Single unilateral BoNT/A injection bilaterally reduced IoNC induced dural extravasation, as well as allodynia (lasting more than 2 weeks). Similarly, BoNT/A reduced formalin-induced pain and dural extravasation. Effects of BoNT/A on pain and dural extravasation in IoNC model were dependent on axonal transport through sensory neurons, as evidenced by colchicine injections (5 mM, 2 µl) into the trigeminal ganglion completely preventing BoNT/A effects. Two different types of pain, IoNC and formalin, are accompanied by dural extravasation. The lasting effect of a unilateral injection of BoNT/A in experimental animals suggests that BoNT/A might have a long-term beneficial effect in craniofacial pain associated with dural neurogenic inflammation. Bilateral effects of BoNT/A and dependence on retrograde axonal transport suggest a central site of its action.Keywords
This publication has 46 references indexed in Scilit:
- Preclinical pharmacological profile of the selective 5-HT1F receptor agonist lasmiditanCephalalgia, 2010
- Lasting reduction of postsurgical hyperalgesia after single injection of botulinum toxin type A in ratFundamental & Clinical Pharmacology, 2010
- Synaptobrevin I mediates exocytosis of CGRP from sensory neurons and inhibition by botulinum toxins reflects their anti-nociceptive potentialJournal of Cell Science, 2007
- Neuron–Glia Signaling in Trigeminal Ganglion: Implications for Migraine PathologyHeadache: The Journal of Head and Face Pain, 2007
- Anti-allodynic efficacy of botulinum neurotoxin A in a model of neuropathic painNeuroscience, 2007
- Botulinum toxin type A in experimental neuropathic painJournal of Neural Transmission, 2005
- The orofacial formalin testNeuroscience & Biobehavioral Reviews, 2004
- Evidence for 5-HT1B/1D Receptors Mediating the Antimigraine Effect of Sumatriptan and DihydroergotamineCephalalgia, 1991
- Ergot alkaloids block neurogenic extravasation in dura mater: Proposed action in vascular headachesAnnals of Neurology, 1988
- A Surgical Approach to the Ophthalmic-Maxillary Nerve Trunks in the RatJournal of Dental Research, 1973