QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION
Open Access
- 1 August 1933
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 58 (2), 137-152
- https://doi.org/10.1084/jem.58.2.137
Abstract
1. The preparation is described of a deep red protein dye, R-salt-azo-benzidine-azo-crystalline egg albumen, which contains no more than traces of protein with the original egg albumen specificity. 2. Based on previous publications of the writers, a quantitative method is given for the micro estimation of precipitin in the antisera to the dye. The method gives the actual weight of precipitin and may be applied to the determination of the maximum amount of precipitable antibody in any antiserum. 3. Data are given (1) on the influence of the period between the final injection and the bleeding on the precipitin content of rabbit antisera to the azo protein; (2) on the magnitude of the antibody response following the injection of multiple doses of the antigen varying within wide limits; (3) on the variations in the precipitin content of the sera of rabbits given successive courses of antigen injections; and (4) on the stability of antisera stored in the cold. 4. Four antisera were obtained in which over 100 times as much precipitin was recovered as the amount of antigen injected. This supplements the growing mass of evidence against the theory that specific antigen fragments are actually incorporated into the antibody molecule.This publication has 7 references indexed in Scilit:
- THE CELLULAR REACTIONS TO LIPOID FRACTIONS FROM ACID-FAST BACILLIThe Journal of Experimental Medicine, 1932
- SEROLOGICAL REACTIONS WITH SIMPLE CHEMICAL COMPOUNDS (PRECIPITIN REACTIONS)The Journal of Experimental Medicine, 1932
- QUANTITATIVE STUDIES ON THE PRECIPITIN REACTIONThe Journal of Experimental Medicine, 1932
- CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINSThe Journal of Experimental Medicine, 1931
- SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERAThe Journal of Experimental Medicine, 1930
- Data on a Protein-Antibody SystemScience, 1930
- A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODYThe Journal of Experimental Medicine, 1929