Strong fluorescence-signal gain with single-excitation-enhancing and emission-directing nanostructured diffraction grating
- 13 October 2009
- journal article
- Published by Optica Publishing Group in Optics Letters
- Vol. 34 (20), 3208-3210
- https://doi.org/10.1364/ol.34.003208
Abstract
A dielectric subwavelength diffraction grating structure is designed and fabricated in order to enhance fluorescence-based detection of biomolecules. Two separate phenomena, enhancement of the local energy densities of the excitation illumination and direction of the emitted signal toward the detector, are examined theoretically and experimentally. 530-fold enhancement of detected signal is achieved compared with the signal from flat surface. Also, changes in polarization and coherence properties of the fluorescent light are found to be remarkable.Keywords
Funding Information
- Graduate School on Modern Optics and Photonics
- Academy of Finland and the Finnish Foundation
- Technology Promotion
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