d-Alanine Deprivation ofBacillus subtilisTeichoic Acids Is without Effect on Cell Growth and Morphology But Affects the Autolytic Activity
- 1 January 1996
- journal article
- research article
- Published by Mary Ann Liebert Inc in Microbial Drug Resistance
- Vol. 2 (1), 123-129
- https://doi.org/10.1089/mdr.1996.2.123
Abstract
Using insertional inactivation of the different genes of the dlt operon in Bacillus subtilis, we searched for metabolic and morphological changes caused by d-alanine ester deprivation of lipoteichoic acid and wall teichoic acid. There were no alterations of cell growth, basic metabolism, cellular content of phosphorus-containing compounds, ultrastructure, cell separation, and surface charge. The only alteration observed was an enhancement of endogenous and β-lactam-induced cell lysis. Since this enhancement is doubtless correlated with the d-alanine ester deprivation of the teichoic acids, the present view based on in vitro experiments, that negatively charged LTA is inhibitory to autolysins, may be questioned. We propose that negatively charged lipoteichoic acid and/or wall teichoic acid serve in vivo to fix the cationic autolysins within the cell wall–membrane complex by electrostatic interaction. Positively charged d-alanine ester substituents decrease the binding capacity of the teichoic acids for autolysins by charge compensation.Keywords
This publication has 21 references indexed in Scilit:
- ThedltOperon in the Biosynthesis ofd-Alanyl-Lipoteichoic Acid inLactobacillus caseiMicrobial Drug Resistance, 1996
- Small and medium‐angle X‐ray analysis of bacterial lipoteichoic acid phase structureEuropean Journal of Biochemistry, 1991
- Flow microcalorimetry as a tool for an improved analysis of antibiotic activity: The different stages of chloramphenicol actionCellular and Molecular Life Sciences, 1989
- The essential nature of teichoic acids in Bacillus subtilis as revealed by insertional mutagenesisMolecular Genetics and Genomics, 1989
- Alanyl turnover from lipoteichoic acid to teichoic acid inStaphylococcus aureusFEMS Microbiology Letters, 1984
- The alanine ester substitution of lipoteichoic acid (LTA) in Staphylococcus aureusFEBS Letters, 1980
- Prevention of penicillin-induced lysis of Staphylococcus aureus by cellular lipoteichoic acid.The Journal of Antibiotics, 1979
- Occurrence and function of membrane teichoic acidsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1977
- Inhibition of bacterial wall lysins by lipoteichoic acids and related compoundsBiochemical and Biophysical Research Communications, 1975
- Lipoteichoic acid: a specific inhibitor of autolysin activity in Pneumococcus.Proceedings of the National Academy of Sciences, 1975