Bone Mineral Density Changes after Ovariectomy in Rats as an Osteopenic Model : Stepwise Description of Double Dorso-Lateral Approach
Open Access
- 1 January 2010
- journal article
- Published by Korean Neurosurgical Society in Journal of Korean Neurosurgical Society
- Vol. 48 (4), 309-312
- https://doi.org/10.3340/jkns.2010.48.4.309
Abstract
This study describes a method for inducing osteopenia using bilateral ovariectomy (OVX), which causes significant changes in bone mineral density (BMD) in rats. Twenty-five 10-week-old female Sprague Dawley rats were used. Five rats were euthanized after two weeks, and BMD was measured in their femora. The other 20 rats were assigned to one of two groups : a sham group (n = 10), which underwent a sham operation, and an OVX group (n = 10), which underwent bilateral OVX at 12 weeks of age. After six weeks, five rats from each group were euthanized, and BMD was measured in their femora. The same procedures were performed in the remaining rats form each group eight weeks later. The femur BMD was significantly lower in the six-week OVX group than in the six-week sham group, and in the eight-week OVX group than in the eight-week sham group. Bilateral OVX is a safe method for creating an osteopenic rat model. The significant decrease in BMD appears six weeks after bilateral OVX.Keywords
This publication has 15 references indexed in Scilit:
- Ovariectomy‐associated changes in bone mineral density and bone marrow haematopoiesis in ratsInternational Journal of Experimental Pathology, 2009
- The effect of anti-resorptive therapies on bone graft healing in an ovariectomized rat spinal arthrodesis modelBone, 2008
- Effects of ibandronate on bone quality: Preclinical studiesBone, 2007
- Available animal models of osteopenia — Small and largeBone, 1995
- FDA guidelines and animal models for osteoporosisBone, 1995
- Identification of an at risk group for osteoporosis using heel ultrasound measurement in primary careBone, 1995
- The Role of Fusion and Instrumentation in the Treatment of Degenerative Spondylolisthesis with Spinal StenosisPublished by Ovid Technologies (Wolters Kluwer Health) ,1993
- The Use of Primary Internal Fixation in SpondylolisthesisSpine, 1993
- A Prospective, Randomized Study of Lumbar FusionSpine, 1993
- The ovariectomized rat model of postmenopausal bone lossBone and Mineral, 1991