Intracellular pH Measured in Isolated Normal Human Leucocytes, by Using 5,5-Dimethyl-2,4-Oxazolidinedione under Conditions of Varying Extracellular Pco2 and Bicarbonate

Abstract

1. Leucocytes were separated from human peripheral blood and incubated under conditions of constant extracellular pH (about 7·40) but variable extracellular Pco₂ and bicarbonate concentration. Intracellular pH of leucocytes was measured by the 5,5-dimethyl-2[¹⁴C],4-oxazolidinedione procedure. 2. At Pco₂ 40 mmHg, the intracellular pH was 7·104 ± 0·115 (mean±SD). At Pco₂ 15 mmHg, intracellular pH was 7·567 ± 0·084, and at Pco₂ 72 mmHg it was 7·098 ± 0·063.