Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast
- 1 February 1998
- journal article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 16 (2), 186-189
- https://doi.org/10.1038/nbt0298-186
Abstract
The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the delta 22-desaturase gene and introduction of the Arabidopsis thaliana delta 7-reductase activity and coexpression of bovine side chain cleavage cytochrome P450, adrenodoxin, and adrenodoxin reductase, lead to pregnenolone biosynthesis from a simple carbon source. Following additional coexpression of human 3 beta-hydroxysteroid dehydrogenase/isomerase, pregnenolone is further metabolized to progesterone. Steroid formation appears to be coupled to yeast sterol biosynthesis.Keywords
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