Determination of Thymosin β4 and Protein in Human Wound Fluid after Abdominal Surgery
- 1 September 2007
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1112 (1), 418-424
- https://doi.org/10.1196/annals.1415.023
Abstract
Wound fluids were collected up to 60 h after abdominal surgery. Immediately after obtaining the wound fluid by Robinson drainage, wound fluid was centrifuged to remove blood cells and inflammatory cells. The concentration of total protein as well as of thymosin beta(4) was determined in the cell-free supernatant solution. Total protein concentration decreased from about 50 g/L to 30 g/L within 60 h after surgery. After surgery we observed a concentration of up to 20 mg thymosin beta(4) per liter decreasing to about 1 mg/L with time. Neither thymosin beta(10) nor oxidized thymosin beta(4) was detected in human wound fluid.Keywords
This publication has 14 references indexed in Scilit:
- In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomicsProteomics, 2006
- Thymosin β: actin-sequestering protein moonlights to repair injured tissuesTrends in Molecular Medicine, 2005
- Factor XIIIa Incorporates Thymosin β4 Preferentially into the Fibrin(ogen) αC-DomainsBiochemistry, 2004
- Thymosin β4is released from human blood platelets and attached by factor XIIIa (transglutaminase) to fibrin and collagenThe FASEB Journal, 2002
- β-Thymosins, small acidic peptides with multiple functionsThe International Journal of Biochemistry & Cell Biology, 2001
- Thymosin β4 serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescent dansylcadaverine does not abolish interaction with G‐actin1FEBS Letters, 1999
- HPLC and post-column derivatization with fluorescamine: isolation of actin-sequestering β-thymosins by reversed-phase HPLCAnalytica Chimica Acta, 1997
- Oxidation and reduction of thymosin β4 and its influence on the interaction with G-actin studied by reverse-phase HPLC and post-column derivatization with fluorescamineAnalytica Chimica Acta, 1997
- Biochemical and Antibacterial Analysis of Human Wound and Blister FluidEuropean Journal of Biochemistry, 1996
- Determination of thymosin β4 in human blood cells and serumJournal of Chromatography A, 1987