Regulation of sperm function by reactive oxygen species
Open Access
- 1 September 2004
- journal article
- review article
- Published by Oxford University Press (OUP) in Human Reproduction Update
- Vol. 10 (5), 387-399
- https://doi.org/10.1093/humupd/dmh034
Abstract
Sperm capacitation can be increased by the addition of reactive oxygen species (ROS) and decreased by antioxidants. Broadly consistent results have been achieved with a wide variety of methods and across different species. Exposure to ROS increases protein tyrosine phosphorylation consequent on an increase in cAMP and activation of tyrosine kinase and inhibition of tyrosine phosphatase. The measurement of ROS production by sperm is complicated by contamination of suspensions by leukocytes, laying many studies open to doubt. In human sperm the observation that extracellular NADPH could support superoxide production detected with the chemiluminescent probe lucigenin and had physiological effects similar to hydrogen peroxide led to the suggestion that they contained NADPH oxidase activity to generate ROS to support capacitation. However, the realization that lucigenin can signal superoxide artefactually, combined with failure to detect superoxide production using spin trapping techniques or to detect NADPH oxidase components in mature sperm, and confirmation of old reports that NADPH solution contains substantial amounts of hydrogen peroxide due to autoxidation, have undermined this hypothesis. Although the presence of significant NADPH oxidase activity in mature human sperm now seems less likely, other observations continue to suggest that they can make ROS in some way. There is stronger evidence that animal sperm can make ROS although these may be mainly of mitochondrial origin.Keywords
This publication has 120 references indexed in Scilit:
- Redox activity associated with the maturation and capacitation of mammalian spermatozoaFree Radical Biology & Medicine, 2004
- Endogenous Redox Activity in Mouse Spermatozoa and Its Role in Regulating the Tyrosine Phosphorylation Events Associated with Sperm Capacitation1Biology of Reproduction, 2003
- Regulation of the Human Sperm Tyrosine Kinase c-yes. Activation by Cyclic Adenosine 3′,5′-Monophosphate and Inhibition by Ca2+ 1Biology of Reproduction, 2002
- Alteration of Glutathione Reductase Expression in the Female Reproductive Organs During the Estrous Cycle1Biology of Reproduction, 2001
- Identification and Functional Analysis of Splice Variants of the Germ Cell Soluble Adenylyl CyclaseJournal of Biological Chemistry, 2001
- Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation, and the presence of leukocytes and precursor germ cells in human sperm suspensionsMolecular Reproduction and Development, 1994
- Inverse relationship between the induction of human sperm capacitation and spontaneous acrosome reaction by various biological fluids and the superoxide scavenging capacity of these fluidsInternational Journal of Andrology, 1993
- Human sperm hyperactivation and capacitation as parts of an oxidative process☆Free Radical Biology & Medicine, 1993
- Reactive oxygen species and human spermatozoa: Analysis of the cellular mechanisms involved in luminol‐ and lucigenin‐dependent chemiluminescenceJournal of Cellular Physiology, 1992
- Production of Superoxide and Activity of Superoxide Dismutase in Rabbit Epididymal SpermatozoaBiology of Reproduction, 1982