Two barley (Hordeum vulgare L.) cultivars, Charlottetown 80 and Herta, were grown on plots of Tormentine sandy loam soil which had been limed to a range of pH levels (4.8, 5.1, 5.8, 6.6 and 7.1) for 10 years previous to this experiment. The pH areas were subdivided for a factorial arrangement of N fertilizer at 30, 60, 90 and 120 kg/ha with P-K fertilizer at 10–19, 30–56, 59–112 and 88–168 kg/ha. Yield of each cultivar increased when the soil pH was increased from 4.8 to 5.8. The Charlottetown cultivar exceeded Herta in yield at the low pH levels. The greatest response from N fertilizer was at the 60 and 90 kg rate in combination with P-K at 59–112 and 88–168 kg per ha, for the mean of the two cultivars. Percent N in barley tissue varied with pH and decreased with increasing rate of P-K fertilization. Percent total N and protein nitrogen in barley grain increased as pH increased. Percent P and K in tissue also increased as the pH increased. In vitro digestibility of barley grain was not affected by P-K, but decreased slightly as N fertility increased.