Alteration of Bone Metabolism in Tissue Culture in Response to Parathyroid Extract.

Abstract

Summary The metabolism of mouse calvaria grown in tissue culture is markedly altered by parathyroid hormone. Accompanying the resorption of bone in the parathyroid hormone treated culture is an increased production of citrate. An enhanced production of lactate occurred during the first 4 days of the experiment which appeared to reflect an increased utilization of glucose during this period. Increased amounts of hydroxyproline were also found in the media indicating that the organic matrix was being destroyed. These results are consistent with the theory that the mobilization of mineral during bone resorption is brought about by the increased accumulation of metabolic acids. Of the 2 acids examined here, citrate would appear to play a greater role since it more closely paralleled mineral dissolution.