Color to grayscale staining pattern representation in IIF
- 1 June 2011
- conference paper
- conference paper
- Published by Institute of Electrical and Electronics Engineers (IEEE)
- No. 10637125,p. 1-6
- https://doi.org/10.1109/cbms.2011.5999110
Abstract
Indirect immunofluorescence (IIF) is the recommended technique to detect rheumatic diseases through the analysis of images exhibiting a fluorescence in the green band. Since the request of such tests has recently increased, researches efforts have been directed towards the development of computer-aided diagnosis (CAD) tools supporting the specialists and improving the standardization of the method. Technological advances have made available color cameras for IIF image acquisition, but their use requires to determine which color to greyscale conversion method provides most useful information for the needs of CAD development. In this respect, we experimentally compare four different methods converting a color image into a greyscale one, analyzing wide features sets for each conversion method and applying four classification paradigms. Experiments have been carried out on an annotated dataset of HEp-2 cells, finding out a subset of features which is independent from the color model used and showing that a greyscale representation based on the HSI model better exploits information for IIF images analysis.Keywords
This publication has 21 references indexed in Scilit:
- A decision support system for Crithidia Luciliae image classificationArtificial Intelligence in Medicine, 2011
- Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence testsArthritis Research & Therapy, 2010
- Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cellsAutoimmunity Reviews, 2009
- A multiple expert system for classifying fluorescent intensity in antinuclear autoantibodies analysisPattern Analysis and Applications, 2008
- Indirect immunofluorescence in autoimmune diseases: Assessment of digital images for diagnostic purposeCytometry Part B: Clinical Cytometry, 2007
- Objective quality evaluation of fluorescence images to optimize automatic image acquisitionCytometry Part A, 2006
- Integrated wavelets for enhancement of microcalcifications in digital mammographyIEEE Transactions on Medical Imaging, 2003
- Multiresolution gray-scale and rotation invariant texture classification with local binary patternsIEEE Transactions on Pattern Analysis and Machine Intelligence, 2002
- Simple estimation methods for the Helmholtz?Kohlrausch effectColor Research & Application, 1997
- Invariant image recognition by Zernike momentsIEEE Transactions on Pattern Analysis and Machine Intelligence, 1990