Development and validation of a rapid, aldehyde dehydrogenase bright–based cord blood potency assay
- 12 May 2016
- journal article
- Published by American Society of Hematology in Blood
- Vol. 127 (19), 2346-2354
- https://doi.org/10.1182/blood-2015-08-666990
Abstract
Key Points Cord blood content of ALDHbr cells correlates well with CFUs and may act as a surrogate potency assay for cord blood units. ALDHbr cells in segments are assayed rapidly, allowing potency results to be used for release of the unit from a public cord blood bank.Keywords
This publication has 40 references indexed in Scilit:
- The Assessment of Parameters Affecting the Quality of Cord Blood by the Appliance of the Annexin V Staining Method and Correlation with CFU AssaysStem Cells International, 2013
- Comparison of pre‐cryopreserved and post‐thaw‐and‐wash‐nucleated cell count on major outcomes following unrelated cord blood transplant in childrenPediatric Transplantation, 2012
- The Cord Blood Apgar: a novel scoring system to optimize selection of banked cord blood grafts for transplantation (CME)Transfusion, 2011
- Influence of infused cell dose and HLA match on engraftment after double-unit cord blood allograftsBlood, 2011
- Progenitor cell dose determines the pace and completeness of engraftment in a xenograft model for cord blood transplantationBlood, 2010
- Electronic volume, aldehyde dehydrogenase, and stem cell marker expression in cells from human peripheral blood apheresis samplesCytometry Part B: Clinical Cytometry, 2010
- Cord blood banking for clinical transplantationBone Marrow Transplantation, 2009
- Results of the Cord Blood Transplantation Study (COBLT): clinical outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with hematologic malignanciesBlood, 2008
- Extended flow cytometry characterization of normal bone marrow progenitor cells by simultaneous detection of aldehyde dehydrogenase and early hematopoietic antigens: implication for erythroid differentiation studiesBMC Physiology, 2008
- Placental Blood as a Source of Hematopoietic Stem Cells for Transplantation into Unrelated RecipientsNew England Journal of Medicine, 1996