ALTERATIONS IN THE EXPRESSION OF A HEPATOCYTE CELL-ADHESION MOLECULE BY TRANSPLANTABLE RAT HEPATOCELLULAR CARCINOMAS

Abstract

Alterations in the expression of normal cell surface components on 13 transplantable hepatocellular carcinomas were examined using a heteroantiserum [anti-MW 105,000 glycoprotein (gp105)] reactive with a family of 9 wheat germ agglutinin binding components from normal rat hepatocytes with an average MW of 105,000. Analysis by 2-dimensional polyacrylamide gel electrophoresis of components immunoprecipitated by anti-gp105 antiserum from detergent extracts of transplantable hepatocellular carcinoma cells surface labeled with 125I revealed qualitative and quantitative changes in the expression of anti-gp105-reactive components with the most consistent change being the apparent loss of a pair of acidic (pI 4.1-4.3) glycoproteins by all 13 transplantable hepatocellular carcinoma lines. One-dimensional peptide maps of fragments produced following digestion with V8 protease indicated that these acidic components were closely related in structure but differed significantly from other anti-gp105-reactive components. Immunodepletion analysis with monoclonal antibodies and heteroantisera reactive with individual components recognized by anti-gp105 antiserum showed that the 2 acidic glycoproteins were antigenically and structurally identical to cell-CAM 105, a MW 105,000 glycoprotein involved in cell-cell adhesion of rat hepatocytes. Antibodies raised against purified cell-CAM 105 were specific in immunoprecipitation assays for the acidic components, strongly inhibited reaggregation of hepatocytes, and displayed no reactivity by indirect immunofluorescence or immunoprecipitation analysis with transplantable hepatocellular carcinoma cells. Major alterations in the expression of cell-CAM 105 may be a consistent feature of the malignant phenotype.