Localization of 5-Nucleotidase and Non-specific Alkaline Phosphatase By Starch Gel Electrophoresis

Abstract

By means of starch gel electrophoresis, 2 alkaline phosphatases were demonstrated, one migrating in the region of [alpha]-globulin and [beta]-lipoprotein and the other with [beta]-globulin. 5-Nucleotidase constituted 60% of the former, but was not present in the latter. Although alkaline phosphatase which migrated with [beta]-globulin was elevated in both diseases of bone and liver, it is thought to be derived from bone since it had no 5-nucleotidase. Since 5-nucleotidase is not contained in bone and is not elevated in diseases of the bone, it is thought to be derived from the liver.