Fluorescence nanoscopy in cell biology
Top Cited Papers
- 6 September 2017
- journal article
- review article
- Published by Springer Science and Business Media LLC in Nature Reviews Molecular Cell Biology
- Vol. 18 (11), 685-701
- https://doi.org/10.1038/nrm.2017.71
Abstract
Fluorescence nanoscopy uniquely combines minimally invasive optical access to the internal nanoscale structure and dynamics of cells and tissues with molecular detection specificity. While the basic physical principles of 'super-resolution' imaging were discovered in the 1990s, with initial experimental demonstrations following in 2000, the broad application of super-resolution imaging to address cell-biological questions has only more recently emerged. Nanoscopy approaches have begun to facilitate discoveries in cell biology and to add new knowledge. One current direction for method improvement is the ambition to quantitatively account for each molecule under investigation and assess true molecular colocalization patterns via multi-colour analyses. In pursuing this goal, the labelling of individual molecules to enable their visualization has emerged as a central challenge. Extending nanoscale imaging into (sliced) tissue and whole-animal contexts is a further goal. In this Review we describe the successes to date and discuss current obstacles and possibilities for further development.Keywords
This publication has 222 references indexed in Scilit:
- STED Microscopy with Optimized Labeling Density Reveals 9-Fold Arrangement of a Centriole ProteinBiophysical Journal, 2012
- Imaging Nanometer-Sized α-Synuclein Aggregates by Superresolution Fluorescence Localization MicroscopyBiophysical Journal, 2012
- STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living CellsBiophysical Journal, 2011
- Diffraction-unlimited all-optical imaging and writing with a photochromic GFPNature, 2011
- A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switchingNature Biotechnology, 2011
- STED Nanoscopy of Actin Dynamics in Synapses Deep Inside Living Brain SlicesBiophysical Journal, 2011
- Superresolution Imaging of Chemical Synapses in the BrainNeuron, 2010
- Nanoscale architecture of integrin-based cell adhesionsNature, 2010
- A spindle-like apparatus guides bacterial chromosome segregationNature, 2010
- Toward fluorescence nanoscopyNature Biotechnology, 2003