Oxidative and Phosphorylative Activities of Mitochondria Isolated from Pea Root Tissues

Abstract
Studies of oxidative activity and oxidative phosphorylation in mitochondria from root tissues of pea seedlings revealed high respiratory rates with succinate, [alpha]-ketoglutarate, and L-glutamate. Mitochondria showed respiratory control ratios of 2-3, and ADP/O ratios of 1 with succinate and 2 with glutamate. P/O ratios were also measured. The oxidation of L-glutamate was stereo-specific and followed Michaelis-Menten kinetics. Studies with arsenite and values for R.Q. indicate that conversion to [alpha]-ketoglutarate via glutamic dehydrogenase is the initial reaction in glutamate oxidation.