Analysis of α-Ketocarboxylic Acids by Ion Exchange Hplc With UV and Amperometric Detection
- 1 January 1981
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography
- Vol. 4 (7), 1219-1224
- https://doi.org/10.1080/01483918108068807
Abstract
Cation exchange high performance liquid chromatography with tandem 200nm spectrophotometric and +1.15V amperometric detection was used for the analysis of several .alpha.-ketocarboxylic acids (.alpha.-ketoglutaric acid, .alpha.-ketobutyric acid, .alpha.-ketovaleric acid and .beta.-phenylpyruvic acid). Derivatization of the .alpha.-ketocarboxylic acids was unnecessary. This technique was operable at 2.0 .mu.g/ml concentrations of .alpha.-ketocarboxylic acids. The sensitivity limit was not investigated.Keywords
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