Methods for construction of adenovirus vectors
- 1 April 1995
- journal article
- Published by Springer Science and Business Media LLC in Molecular Biotechnology
- Vol. 3 (3), 207-220
- https://doi.org/10.1007/bf02789331
Abstract
Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow to high titers, and can transduce a variety of cell types in cell culture and in vivo. Vectors can be designed that are either replication competent or replication defective and, in the latter case, are highly efficient at delivering and expressing genes in mammalian cells without resulting in cell killing. Methods are described for growing, titrating, and purifying adenoviruses, for extracting viral DNA from purified virions and from infected cells, for rescuing inserts of foreign DNA into the viral genome, and for assessing expression of inserted genes in adenovirus vectors.Keywords
This publication has 20 references indexed in Scilit:
- Monitoring foreign gene expression by a human adenovirus-based vector using the firefly luciferase gene as a reporterVirus Research, 1993
- Expression of Heterologous Sequences in Adenoviral VectorsPublished by Springer Science and Business Media LLC ,1992
- Use of Human Adenovirus-based Vectors for Antigen Expression in AnimalsJournal of General Virology, 1989
- Expression of the Glycoprotein of Vesicular Stomatitis Virus by Infectious Adenovirus VectorsJournal of General Virology, 1989
- Growth of 293 Cells in Suspension CultureJournal of General Virology, 1987
- Human adenovirus cloning vectors based on infectious bacterial plasmidsGene, 1986
- Covalently closed circles of adenovirus 5 DNANature, 1983
- Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cellsCell, 1979
- Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5Journal of General Virology, 1977
- The infectivity of adenovirus 5 DNA-protein complexVirology, 1976