New saponins, named gypenosides LXI (1), LXII (2), LXIII (3), LXIV (4) and LXVII (5) were isolated from the aerial parts of Gynostemma pentaphyllum MAKINO collected in Miyagi Pref. On the basis of chemical and physicochemical evidence, they were characterized as follows: 1, 2.alpha.,3.beta.,12.beta.,20(S),26-pentahydroxydammar-24-ene 3-O-.beta.-sophoroside-20-O-.beta.-primeveroside; 2, 3.beta.,12.beta.,19,20(S)-tetrahydroxydammar-24-ene 3-O-.beta.-sophoroside-20-O-.beta.-primeveroside; 3, 3.beta.,19,20(S)-trihydroxydammar-24-ene 3-O-.beta.-sophoroside-20-O-.beta.-primeveroside; 4, 3.beta.,12.beta.,19,20(S)-tetrahydroxydammar-24-ene 3-O-.beta.-D-glycopyranoside-20-O-.beta.-primeveroside; 5, 2.alpha.,3.beta.19,20(S)-tetrahydroxydammer-24-ene 3-O-.beta.-sophoroside-20-O-.beta.-primeveroside.