Controlled delivery of platelet‐rich plasma‐derived growth factors for bone formation
- 7 January 2008
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 86A (4), 1128-1136
- https://doi.org/10.1002/jbm.a.31740
Abstract
Platelet-rich plasma (PRP) represents an autologous source of growth factors essential for bone regeneration. The clinical efficacy of PRP is, however, unpredictable, and this is likely due to the inefficient and inconsistent delivery of PRP-derived growth factors. Previous investigations have shown that current methods of PRP preparation result in a premature release of the relevant bone stimulatory factors. As successful bone regeneration requires multiple factors presented in a physiologic temporal and spatial cascade, the objective of this study is to control the bioavailability of PRP-derived growth factors using a hydrogel carrier system. Specifically, the release of platelet-derived growth factor, transforming growth factor beta-1, and insulin-like growth factor from two types of alginate carriers was compared over time. The effects of the released factors on the growth and alkaline phosphatase (ALP) activity of human osteoblast-like cells were also evaluated. It was found that factor release profiles varied as function of carrier type, and binding of growth factors to the alginate matrix also modulated their release. The bioactivity of released factors was maintained in vitro and they promoted cell proliferation and ALP activity. These results demonstrate the potential of this autologous multifactor delivery system for controlling the bioavailability of PRP-derived factors. Future studies will focus on optimizing this system to increase the clinical efficacy of PRP by matching the distribution and temporal sequencing of PRP-derived factors to the bone healing cascade. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2008Keywords
This publication has 41 references indexed in Scilit:
- Applications of Alginate in Bioseparation of ProteinsArtificial Cells, Blood Substitutes, and Immobilization Biotechnology, 2006
- An in vitro evaluation of PCL–TCP composites as delivery systems for platelet-rich plasmaJournal of Controlled Release, 2005
- Bone growth factors in maxillofacial skeletal reconstructionInternational Journal of Oral & Maxillofacial Surgery, 2002
- Birth and Death of Bone Cells: Basic Regulatory Mechanisms and Implications for the Pathogenesis and Treatment of OsteoporosisEndocrine Reviews, 2000
- Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In VitroJournal of Cellular Physiology, 1992
- Alginate as immobilization matrix for cellsTrends in Biotechnology, 1990
- Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beadsBiotechnology & Bioengineering, 1989
- Role of Transforming Growth Factor Beta in Bone Remodeling: A ReviewConnective Tissue Research, 1989
- Growth factors and the regulation of bone remodeling.JCI Insight, 1988
- Biological interactions between polysaccharides and divalent cations: The egg‐box modelFEBS Letters, 1973