Visualizing mechanosensory endings of TrkC‐expressing neurons in HS3ST‐2‐hPLAP mice
- 6 October 2008
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 511 (4), 543-556
- https://doi.org/10.1002/cne.21862
Abstract
Somatosensory neurons are classified into three main types according to their modalities: nociceptive, thermal, and mechanosensory. Within each modality group, neurons can be further divided into morphologically and functionally distinct subclasses. Here we show that heparan sulfate D‐glucosaminyl 3‐O‐sulfotransferase 2 (HS3ST‐2) is a marker for specific subsets of TrkC‐expressing cutaneous low‐threshold mechanosensory and proprioceptive mechanosensory neurons. Two‐color in situ analysis revealed that almost all HS3ST‐2 signals colocalized with TrkC but not with TrkA or TrkB mRNA. To visualize the morphological subtypes of HS3ST‐2/TrkC‐expressing neurons, we generated a HS3ST‐2‐hPLAP knock‐in mouse line, in which HS3ST‐2‐expressing neurons and their projections are labeled by human placental alkaline phosphatase (hPLAP). AP staining in these mice demonstrated that sensory endings of muscle spindles and Golgi tendon organs as well as the cutaneous mechanosensory Merkel and longitudinal lanceolate endings in the whiskers are strongly positive for hPLAP activity. In contrast, no nociceptive endings are labeled. In the glabrous and hairy skin, rare Merkel endings and transverse lanceolate endings are weakly stained. During development, each type of nerve endings forms at different time point. Muscle innervations differentiate first, followed by formation of cutaneous sensory endings. Our results revealed the subtype identities of TrkC‐positive mechanosensory neurons and demonstrated the usefulness of HS3ST‐2 as a genetic marker for these subclasses of neurons. J. Comp. Neurol. 511:543–556, 2008.Keywords
This publication has 61 references indexed in Scilit:
- Retrograde BMP Signaling Regulates Trigeminal Sensory Neuron Identities and the Formation of Precise Face MapsNeuron, 2007
- A Hierarchical NGF Signaling Cascade Controls Ret-Dependent and Ret-Independent Events during Development of Nonpeptidergic DRG NeuronsNeuron, 2007
- The principal neuronal gD-type 3-O-sulfotransferases and their products in central and peripheral nervous system tissues☆Matrix Biology, 2007
- Runx1 selectively regulates cell fate specification and axonal projections of dorsal root ganglion neuronsDevelopmental Biology, 2007
- Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: I. The 3‐O‐sulfotransferase familyDevelopmental Dynamics, 2006
- Overexpression of neurotrophin 4 in skin enhances myelinated sensory endings but does not influence sensory neuron numberJournal of Comparative Neurology, 2006
- Topographically Distinct Epidermal Nociceptive Circuits Revealed by Axonal Tracers Targeted to MrgprdNeuron, 2005
- Visualizing Sexual Dimorphism in the BrainNeuron, 2004
- Biosynthesis of 3-O-sulfated heparan sulfate: unique substrate specificity of heparan sulfate 3-O-sulfotransferase isoform 5Glycobiology, 2003
- The Trk family of neurotrophin receptorsJournal of Neurobiology, 1994