Image Analysis Based Quantification of Bacterial Volume Change with High Hydrostatic Pressure
- 3 November 2008
- journal article
- Published by Wiley in Journal of Food Science
- Vol. 73 (9), M423-M429
- https://doi.org/10.1111/j.1750-3841.2008.00947.x
Abstract
Scanning electron microscopy (SEM) images of Staphylococcus aureus 485 and Escherichia coli O157:H7 933 were taken after pressure treatments at 200 to 400 MPa. Software developed for this purpose was used to analyze SEM images and to calculate the change in view area and volume of cells. Significant increase in average cell view area and volume for S. aureus 485 was observed in response to pressure treatment at 400 MPa. Cell view area for E. coli O157:H7 933 significantly increased at 325 MPa, the maximum pressure treatment tested against this pathogen. In contrast to S. aureus, cells of E. coli O157:H7 exhibited significant increase in average view area and volume at 200 MPa. The pressure-induced increase in these parameters may be attributed to modifications in membrane properties, for example, denaturation of membrane-bound proteins and pressure-induced phase transition of membrane lipid bilayer.Keywords
This publication has 21 references indexed in Scilit:
- Protein unfolding, amyloid fibril formation and configurational energy landscapes under high pressure conditionsChemical Society Reviews, 2006
- Effects of SEM Preparation Techniques on the Appearance of Bacteria and Biofilms in the Carter SandstoneJournal of Sedimentary Research, 2004
- Evaluation of Structural Changes Induced by High Hydrostatic Pressure in Leuconostoc mesenteroidesApplied and Environmental Microbiology, 2004
- Volumetric Properties of ProteinsAnnual Review of Biophysics and Biophysical Chemistry, 2003
- Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiationJournal of Microbiological Methods, 2003
- Effects of Pressure-Induced Membrane Phase Transitions on Inactivation of HorA, an ATP-Dependent Multidrug Resistance Transporter, in Lactobacillus plantarumApplied and Environmental Microbiology, 2002
- Effects of hydrostatic pressure on lipid and surfactant phasesCurrent Opinion in Colloid & Interface Science, 2001
- Evidence for a molten globule-like transition state in protein folding from determination of activation volumesBiochemistry, 1995
- Pressure dependence of 1,6-diphenyl-1,3,5-hexatriene fluorescence in single-component phosphatidylcholine liposomesBiochemistry, 1983
- Cell and organelle shrinkage during preparation for scanning electron microscopy: effects of fixation, dehydration and critical point dryingJournal of Microscopy, 1977