Temporal Variation in Serum Nitrite Levels in Rats and Mice
- 1 January 1999
- journal article
- research article
- Published by Informa UK Limited in Chronobiology International
- Vol. 16 (4), 527-532
- https://doi.org/10.3109/07420529908998726
Abstract
Although considerable evidence implicates involvement of nitric oxide (NO) in circadian regulation, little is known about possible 24 h variations in basal NO metabolism. In this study, daily variations in serum nitrite levels were studied in locally bred mice and rats during the months of September and October. The serum was separated from blood samples obtained at six different times of the day and night (1 h, 5 h, 9 h, 13 h, 17 h, and 21 h after lights off [HALO] from male albino mice and rats). As an index of in vivo NO generation, serum nitrite levels (determined by the diazotization method) in rats exhibited significant temporal fluctuation (unpaired Student t test), with the concentration highest at 5 HALO and 21 HALO and lowest at 9 HALO. No such temporal variation was detected in mice in these studies conducted on locally bred animals in the autumn.Keywords
This publication has 19 references indexed in Scilit:
- Circadian variation of nitric oxide synthase activity and cytosolic protein levels in rat brainBrain Research, 1996
- NADPH diaphorase activity around the suprachiasmatic nucleus in rat brainCell and tissue research, 1996
- Twenty-Four-Hour Variations in the Sensitivity of Rat Aorta to Vasoactive AgentsChronobiology International, 1996
- Distribution of NADPH-diaphorase staining and light-induced fos expression in the rat suprachiasmatic nucleus region supports a role for nitric oxide in the circadian systemNeuroscience, 1995
- Temporal Variation in Hepatic Superoxide Dismutase Activity in MiceChronobiology International, 1995
- Resetting the Biological Clock: Mediation of Nocturnal Circadian Shifts by Glutamate and NOScience, 1994
- Tumor Necrosis Factor and Granulocyte Macrophage-Colony Stimulating Factor Stimulate Human Macrophages to Restrict Growth of Virulent Mycobacterium avium and to Kill Avirulent M. avium: Killing Effector Mechanism Depends on the Generation of Reactive Nitrogen IntermediatesJournal of Leukocyte Biology, 1991
- Glutamate, nitric oxide and cell-cell signalling in the nervous systemTrends in Neurosciences, 1991
- Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediateBiochemistry, 1988
- Analysis of nitrate, nitrite, and [15N]nitrate in biological fluidsAnalytical Biochemistry, 1982