Identification of Metabolites of Trenbolone Acetate in Androgenic Runoff from a Beef Feedlot
- 1 April 2006
- journal article
- Published by Environmental Health Perspectives in Environmental Health Perspectives
- Vol. 114 (Suppl 1), 65-68
- https://doi.org/10.1289/ehp.8055
Abstract
Little is known concerning the potential ecological effects of hormonally active substances associated with discharges from animal feeding operations. Trenbolone acetate is a synthetic anabolic steroid that is widely used in the United States to promote growth of beef cattle. Metabolites of trenbolone acetate include the stereoisomers 17α- and 17β-trenbolone, both of which are stable in animal wastes and are relatively potent androgens in fish and mammals. Our purpose in this study was to evaluate the occurrence of 17α- and 17β-trenbolone in a beef cattle feedlot discharge and in river water upstream and downstream from the discharge. In conjunction with that effort, we measured in vitro androgenic activity of the discharge using CV-1 cells that had been transiently cotransfected with human androgen receptor and reporter gene constructs. Samples were collected on nine different occasions during 2002 and 2003. Whole-water samples from the discharge caused a significant androgenic response in the CV-1 cells and contained detectable concentrations of 17α- and 17β-trenbolone. Further work is needed to ascertain the degree to which synthetic androgens such as trenbolone contribute to androgenic activity of feedlot discharges.Keywords
This publication has 19 references indexed in Scilit:
- Cloning and In Vitro Expression and Characterization of the Androgen Receptor and Isolation of Estrogen Receptor α from the Fathead Minnow (Pimephales promelas)Environmental Science & Technology, 2004
- Survey of hormone activities in municipal biosolids and animal manuresEnvironmental Toxicology and Water Quality, 2004
- Effects of the androgenic growth promoter 17‐β‐trenbolone on fecundity and reproductive endocrinology of the fathead minnowEnvironmental Toxicology and Chemistry, 2003
- Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluentEnvironmental Toxicology and Chemistry, 2002
- Hormones: Here's the BeefScience News, 2002
- The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: environmental studies.Environmental Health Perspectives, 2001
- Biochemistry and physiology of anabolic hormones used for improvement of meat productionAPMIS, 2001
- Androgen Receptor Antagonist versus Agonist Activities of the Fungicide Vinclozolin Relative to HydroxyflutamideJournal of Biological Chemistry, 1995
- THE EFFECTS OF STEROID HORMONES AND XENOBIOTICS ON GROWTH OF FARM ANIMALSPublished by Elsevier BV ,1986
- Differences in the biotransformation of a 17β-hydroxylated steroid, trenbolone acetate, in rat and cowXenobiotica, 1981