Growth of Dehalobacter and Dehalococcoides spp. during Degradation of Chlorinated Ethanes
Open Access
- 1 January 2006
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (1), 428-436
- https://doi.org/10.1128/aem.72.1.428-436.2006
Abstract
Mixed anaerobic microbial subcultures enriched from a multilayered aquifer at a former chlorinated solvent disposal facility in West Louisiana were examined to determine the organism(s) involved in the dechlorination of the toxic compounds 1,2-dichloroethane (1,2-DCA) and 1,1,2-trichloroethane (1,1,2-TCA) to ethene. Sequences phylogenetically related to Dehalobacter and Dehalococcoides , two genera of anaerobic bacteria that are known to respire with chlorinated ethenes, were detected through cloning of bacterial 16S rRNA genes. Denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments after starvation and subsequent reamendment of culture with 1,2-DCA showed that the Dehalobacter sp. grew during the dichloroelimination of 1,2-DCA to ethene, implicating this organism in degradation of 1,2-DCA in these cultures. Species-specific real-time quantitative PCR was further used to monitor proliferation of Dehalobacter and Dehalococcoides during the degradation of chlorinated ethanes and showed that in fact both microorganisms grew simultaneously during the degradation of 1,2-DCA. Conversely, Dehalobacter grew during the dichloroelimination of 1,1,2-TCA to vinyl chloride (VC) but not during the subsequent reductive dechlorination of VC to ethene, whereas Dehalococcoides grew only during the reductive dechlorination of VC but not during the dichloroelimination of 1,1,2-TCA. This demonstrated that in mixed cultures containing multiple dechlorinating microorganisms, these organisms can have either competitive or complementary dechlorination activities, depending on the chloro-organic substrate.Keywords
This publication has 51 references indexed in Scilit:
- Reductive dechlorination of β-hexachlorocyclohexane (β-HCH) by a Dehalobacter species in coculture with a Sedimentibacter sp.FEMS Microbiology Ecology, 2005
- Genetic Identification of a Putative Vinyl Chloride Reductase in Dehalococcoides sp. Strain BAV1Applied and Environmental Microbiology, 2004
- Molecular Identification of the Catabolic Vinyl Chloride Reductase from Dehalococcoides sp. Strain VS and Its Environmental DistributionApplied and Environmental Microbiology, 2004
- Vinyl Chloride and cis-Dichloroethene Dechlorination Kinetics and Microorganism Growth under Substrate Limiting ConditionsEnvironmental Science & Technology, 2004
- Stereoselective Microbial Dehalorespiration with Vicinal Dichlorinated AlkanesApplied and Environmental Microbiology, 2003
- Microbial Dehalorespiration with 1,1,1-TrichloroethaneScience, 2002
- Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropaneFEMS Microbiology Ecology, 2002
- Cometabolism of chlorinated solvents by nitrifying bacteria: Kinetics, substrate interactions, toxicity effects, and bacterial responseBiotechnology & Bioengineering, 1997
- Reductive Dehalogenation of Chlorinated Ethenes and Halogenated Ethanes by a High-Rate Anaerobic Enrichment CultureEnvironmental Science & Technology, 1994
- Reductive Dechlorination Of Tetrachloroethylene by a Chlorobenzoate-Enriched Biofilm ReactorEnvironmental Science & Technology, 1994