Transcriptional Programming and Functional Interactions within the Phytophthora sojae RXLR Effector Repertoire
Open Access
- 1 June 2011
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 23 (6), 2064-2086
- https://doi.org/10.1105/tpc.111.086082
Abstract
The genome of the soybean pathogen Phytophthora sojae contains nearly 400 genes encoding candidate effector proteins carrying the host cell entry motif RXLR-dEER. Here, we report a broad survey of the transcription, variation, and functions of a large sample of the P. sojae candidate effectors. Forty-five (12%) effector genes showed high levels of polymorphism among P. sojae isolates and significant evidence for positive selection. Of 169 effectors tested, most could suppress programmed cell death triggered by BAX, effectors, and/or the PAMP INF1, while several triggered cell death themselves. Among the most strongly expressed effectors, one immediate-early class was highly expressed even prior to infection and was further induced 2- to 10-fold following infection. A second early class, including several that triggered cell death, was weakly expressed prior to infection but induced 20- to 120-fold during the first 12 h of infection. The most strongly expressed immediate-early effectors could suppress the cell death triggered by several early effectors, and most early effectors could suppress INF1-triggered cell death, suggesting the two classes of effectors may target different functional branches of the defense response. In support of this hypothesis, misexpression of key immediate-early and early effectors severely reduced the virulence of P. sojae transformants.Keywords
This publication has 101 references indexed in Scilit:
- Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1Proceedings of the National Academy of Sciences, 2010
- Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestansNature, 2009
- A Major Role of the MEKK1–MKK1/2–MPK4 Pathway in ROS SignallingMolecular Plant, 2009
- MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plantsCell Research, 2008
- RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 membersProceedings of the National Academy of Sciences, 2008
- Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of riceProceedings of the National Academy of Sciences of the United States of America, 2007
- Indirect activation of a plant nucleotide binding site–leucine-rich repeat protein by a bacterial proteaseProceedings of the National Academy of Sciences, 2007
- The plant immune systemNature, 2006
- An ancestral oomycete locus contains late blight avirulence gene Avr3a , encoding a protein that is recognized in the host cytoplasmProceedings of the National Academy of Sciences, 2005
- Specific perception of subnanomolar concentrations of chitin fragments by tomato cells: induction of extracellular alkalinization, changes in protein phosphorylation, and establishment of a refractory stateThe Plant Journal, 1993