Molecular cloning and characterization of Byp, a murine receptor‐type tyrosine phosphatase similar to human DEP‐1
- 2 January 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 378 (1), 7-14
- https://doi.org/10.1016/0014-5793(95)01415-2
Abstract
Novel murine cDNAs encoding a receptor-like protein tyrosine phosphatase, termed Byp (HPTP beta-like tyrosine phosphatase) were cloned. The putative Byp protein consists of 1238 amino acids, which possesses a single catalytic domain in the cytoplasmic region. The extracellular region comprises eight repeats of a fibronectin type III module and contains multiple N-glycosylation sites. The byp mRNA was 7.7-kb long and expressed in every tissue examined, its level being high in the brain and kidney. Transfection of the byp cDNA expression plasmid into COS7 cells resulted in the expression of a 220-kDa tyrosine phosphorylated protein. Furthermore, co-expression of Byp and the Src family kinase Fyn increased the level of tyrosine phosphorylation of Byp, suggesting that Byp was tyrosine-phosphorylated by Fyn. Finally, the byp gene was located to mouse chromosome 2E1–2E2 and rat chromosome 3832–3833.Keywords
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