Large-Scale Production of Coenzyme F 420 -5,6 by Using Mycobacterium smegmatis
Open Access
- 1 November 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (11), 5750-5755
- https://doi.org/10.1128/aem.68.11.5750-5755.2002
Abstract
Production of coenzyme F 420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F 420 -5,6. M . smegmatis produced 1 to 3 μmol of intracellular F 420 per liter of culture, which was more than the 0.85 to 1.0 μmol of F 420 -2 per liter usually obtained with Methanobacterium thermoautotrophicum and ∼10-fold higher than what was previously reported for the best aerobic actinomycetes. An improved chromatography system using rapidly flowing quaternary aminoethyl ion-exchange material and Florisil was used to more quickly and easily purify F 420 than with previous methods.Keywords
This publication has 36 references indexed in Scilit:
- Molecular characterization and analysis of the biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae NRRL 2564Cell Chemical Biology, 1999
- Presence of F420-dependent glucose-6-phosphate dehydrogenase in Mycobacterium and Nocardia species, but absence from Streptomyces and Corynebacterium species and methanogenic ArchaeaFEMS Microbiology Letters, 1997
- Mycobacterium phlei Infection of the Foot: A Case ReportFoot & Ankle International, 1994
- F420H2: quinone oxidoreductase from Archaeoglobus fulgidusEuropean Journal of Biochemistry, 1994
- Tuberculosis: Dr. Enarson repliesMayo Clinic Proceedings, 1994
- Purification and properties of N5, N10‐methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg)European Journal of Biochemistry, 1990
- Mycobacterial Infections in. AIDS Patients, with an Emphasis on the Mycobacterium avium ComplexClinical Infectious Diseases, 1986
- Coenzyme F420 dependence of the methylenetetrahydromethanopterin dehydrogenase of MethanobacteriumthermoautotrophicumBiochemical and Biophysical Research Communications, 1985
- PHOTOREACTIVATING ENZYME FROM STREPTOMYCES GRISEUS—III. EVIDENCE FOR THE PRESENCE OF AN INTRINSIC CHROMOPHOREPhotochemistry and Photobiology, 1980
- Synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin and confirmation of its identity with the deazaisoalloxazine chromophore of Methanobacterium redox coenzyme F420Journal of the American Chemical Society, 1979