Cytokine Expression of Cord and Adult Blood Mononuclear Cells in Response to Streptococcus agalactiae
- 1 March 2002
- journal article
- Published by Springer Science and Business Media LLC in Pediatric Research
- Vol. 51 (3), 304-309
- https://doi.org/10.1203/00006450-200203000-00007
Abstract
Neonatal bacterial sepsis is often characterized by a fulminant clinical course and highly elevated plasma levels of proinflammatory cytokines. To evaluate in vitro activation of the neonatal immune system by specific infectious stimuli, cord blood cells from healthy neonates were examined for expression of tumor necrosis factor-α (TNF-α), IL-1β, IL-6, and IL-8 in response to Streptococcus agalactiae (GBS), lipopolysaccharide (LPS), and lipoteichoic acid (LTA). Cytokine-expression was compared in mononuclear cells from cord and adult peripheral blood. TNF-α and IL-6 levels in the supernatant of cord blood cell cultures were significantly higher after stimulation with heat-killed GBS (107/mL) than with LPS (2 μg/mL) or LTA (2 μg/mL) (TNF-α: 2215 versus 267.5 versus 40 pg/mL, p = 0.001; IL-6: 9667 versus 4909 versus 919 pg/mL, p = 0.006). mRNA expression of TNF-α, IL-1β, IL-6, and IL-8 was equally pronounced after stimulation with either GBS, LPS, or LTA in cord or adult blood cells at various times. A MAb directed against the monocyte receptor molecule CD14 did not inhibit the release of cytokines in cord blood mononuclear cells after stimulation with GBS. In summary, activation of cord blood cells by infectious stimuli is comparable to the adult immune response in terms of expression of proinflammatory cytokines. GBS in particular proves to be a potent activator of the neonatal immune system when compared with LPS and LTA. CD14 seems not to be a crucial molecule for activation of cord blood cells by GBS.Keywords
This publication has 37 references indexed in Scilit:
- Human Monocyte Receptors Involved in Tumor Necrosis Factor Responses to Group B Streptococcal ProductsInfection and Immunity, 2000
- Intracellular and Extracellular Cytokine Production by Human Mixed Mononuclear Cells in Response to Group B StreptococciInfection and Immunity, 2000
- Predisposing conditions and pathogens in bacteremia in hospitalized childrenEuropean Journal of Clinical Microbiology & Infectious Diseases, 1998
- Regulation of decidual cell chemokine production by group B streptococci and purified bacterial cell wall componentsAmerican Journal of Obstetrics and Gynecology, 1997
- Macrophages sense pathogens via DNA motifs: induction of tumor necrosis factor‐α‐mediated shockEuropean Journal of Immunology, 1997
- Interleukin-6 Production by Human Neonatal Monocytes Stimulated by Type III Group B StreptococciThe Journal of Infectious Diseases, 1996
- Neonatal Tolerance Revisited: Turning on Newborn T Cells with Dendritic CellsScience, 1996
- Group B Streptococci Elicit Leukotriene B4 and Interleukin-8 from Human Monocytes: Neonates Exhibit a Diminished ResponseThe Journal of Infectious Diseases, 1995
- Production of tumor necrosis factor by human cells in vitro and in vivo, induced by group B streptococciThe Journal of Pediatrics, 1993
- A ten-year review of neonatal sepsis and comparison with the previous fifty-year experienceThe Pediatric Infectious Disease Journal, 1990