Silica‐immobilized enzymes for multi‐step synthesis in microfluidic devices
- 5 April 2007
- journal article
- other
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 98 (3), 701-705
- https://doi.org/10.1002/bit.21447
Abstract
The combinatorial synthesis of 2-aminophenoxazin-3-one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica-immobilized hydroxylaminobenzene mutase and silica-immobilized soybean peroxidase are connected in series to create a chemo-enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2-aminophenol, followed by enzymatic polymerization to APO. Silica-immobilization of enzymes allows the rapid stabilization and integration of the biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions. Biotechnol. Bioeng. 2007;98: 701–705.Keywords
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