Simian Virus 40 Integration Site in an Adenovirus 7-Simian Virus 40 Hybrid DNA Molecule
- 1 May 1971
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 68 (5), 1037-1041
- https://doi.org/10.1073/pnas.68.5.1037
Abstract
The E46+ strain of Adenovirus 7 is a mixed-virus population containing defective Adenoviurs 7-SV40 hybrid particles and helper, nonhybrid Adenovirus 7 particles. We have applied electron microscopic mapping techniques to obtain a physical map of the genome of the hybrid particles present in E46+PL1, a substrain of E46+ derived from a single two-hit plaque. DNA molecules extracted from purified E46+Pl1 virions were found to be linear duplexes, with a mean lenght of 10.9 μm. When these molecules were denatured and renatured, a unique heteroduplex was formed that presumably derived one of its strands from an Adenovirus 7-SV40 hybrid molecule and the other from a nonhybrid Adenovirus 7 molecule. This heteroduplex was double-stranded, except for a short region near one end where the two strands were not paired. On the basis of measurements of the lengths of the single-and double-stranded regions in the heteroduplex, the structure of the Adenovirus 7-SV40 hybrid genome can be reconstructed as follows: The hybrid genome contains 16% less Adenovirus 7 DNA than the nonhybrid Adenovirus 7 genome. This deletion consists of the segment of DNA that maps between 0.05 and 0.21 molecular lenghts in the nonhybrid Adenovirus 7 DNA molecule. The deleted DNA has been partially replaced by an amount of heterologous DNA equivalent to 75% of the complete SV40 genome. A model for the generation of the hybrid genome is presented.Keywords
This publication has 15 references indexed in Scilit:
- Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopyJournal of Molecular Biology, 1970
- Mapping of Deletions and Substitutions in Heteroduplex DNA Molecules of Bacteriophage Lambda by Electron MicroscopyScience, 1969
- The Arrangement of Information in DNA MoleculesThe Journal of general physiology, 1966
- Studies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials.Proceedings of the National Academy of Sciences, 1966
- RNA production in adenovirus-infected KB cellsVirology, 1965
- STUDIES OF ADENOVIRUS SV40 HYBRID VIRUSESThe Journal of Experimental Medicine, 1965
- THE INCORPORATION OF SV40 GENETIC MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGENProceedings of the National Academy of Sciences of the United States of America, 1964
- EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSESProceedings of the National Academy of Sciences of the United States of America, 1964
- INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUSProceedings of the National Academy of Sciences of the United States of America, 1964
- Biochemical studies on adenovirus multiplicationVirology, 1963