Displacement fractionation of deoxyribonucleoproteins by heparin and dextran sulphate
- 1 April 1958
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 68 (4), 568-572
- https://doi.org/10.1042/bj0680568
Abstract
Histones form conjugates with Na heparinate and with dextran sulfate which are insoluble in 0.14 [image] NaCl. Stepwise addition of subequivalent quantities of these polysulfates to undenatured deoxyribonucleohistone preparations results in the liberation of deoxyribonucleic acid fractions. The fractions from calf thymus have base ratios (adenine plus thy mine: guanine plus cytosine) in the range 0.78-1.21, and those from ox spleen have values between 0.93 and 1.40. Results suggest that all the histone is combined with deoxyribonucleic acid in isolated deoxyribonucleo-protein.Keywords
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