Molecular analysis of fiber type-specific expression of murine myostatin promoter
- 1 October 2004
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 287 (4), C1031-C1040
- https://doi.org/10.1152/ajpcell.00492.2003
Abstract
Myostatin is a negative regulator of muscle growth, and absence of the functional myostatin protein leads to the heavy muscle phenotype in both mouse and cattle. Although the role of myostatin in controlling muscle mass is established, little is known of the mechanisms regulating the expression of the myostatin gene. In this study, we have characterized the murine myostatin promoter in vivo. Various constructs of the murine myostatin promoter were injected into the quadriceps muscle of mice, and the reporter luciferase activity was analyzed. The results indicate that of the seven E-boxes present in the 2.5-kb fragment of the murine myostatin promoter, the E5 E-box plays an important role in the regulation of promoter activity in vivo. Furthermore, the in vitro studies demonstrated that MyoD preferentially binds and upregulates the murine myostatin promoter activity. We also analyzed the activity of the bovine and murine promoters in murine skeletal muscle and showed that, despite displaying comparable levels of activity in murine myoblast cultures, bovine myostatin promoter activity is much weaker than murine myostatin promoter in mice. Finally, we demonstrate that in vivo, the 2.5-kb region of the murine myostatin promoter is sufficient to drive the activity of the reporter gene in a fiber type-specific manner.Keywords
This publication has 38 references indexed in Scilit:
- Tenotomy decreases reporter protein synthesis via the 3′-untranslated region of the β-myosin heavy chain mRNAAmerican Journal of Physiology-Cell Physiology, 2000
- Skeletal muscle myostatin mRNA expression is fiber-type specific and increases during hindlimb unloadingAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1999
- Fiber-Type-Specific Transcription of the Troponin I Slow Gene Is Regulated by Multiple ElementsMolecular and Cellular Biology, 1999
- Preferential Binding of MyoD-E12 versus Myogenin-E12 to the Murine Sarcoma Virus Enhancer in VitroPublished by Elsevier BV ,1996
- Muscle Gene E-box Control ElementsPublished by Elsevier BV ,1995
- A Skeletal Muscle-Specific Enhancer Regulated by Factors Binding to E and CArG Boxes Is Present in the Promoter of the Mouse Myosin Light-Chain 1A GeneMolecular and Cellular Biology, 1995
- Delineation of a slow-twitch-myofiber-specific transcriptional element by using in vivo somatic gene transfer.Proceedings of the National Academy of Sciences of the United States of America, 1995
- Positive control mutations in the MyoD basic region fail to show cooperative DNA binding and transcriptional activation in vitro.Proceedings of the National Academy of Sciences of the United States of America, 1994
- Multiple regulatory elements contribute differentially to muscle creatine kinase enhancer activity in skeletal and cardiac muscle.Molecular and Cellular Biology, 1993
- Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study.Proceedings of the National Academy of Sciences, 1988