Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin β2 subunit
- 20 August 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 505 (1), 27-30
- https://doi.org/10.1016/s0014-5793(01)02778-8
Abstract
The cysteine-rich region (CRR) of the integrin β subunits is organised into four repeating elements. By expression of a panel of truncated β2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482–Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of ‘xC---C---C---CxCxxCxC---Cx’, where ‘x’ represents a single residue, and ‘---’ represents a stretch of four to 14 residues.Keywords
This publication has 23 references indexed in Scilit:
- Amino Acid Residues in the PSI Domain and Cysteine-rich Repeats of the Integrin β2 Subunit That Restrain Activation of the Integrin αxβ2Journal of Biological Chemistry, 2001
- Human Leukocytes Contain a Large Pool of Free Forms of CD18Biochemical and Biophysical Research Communications, 2000
- Structural and Functional Studies with Antibodies to the Integrin β2 SubunitPublished by Elsevier BV ,2000
- The Structure of the β-Propeller Domain and C-terminal Region of the Integrin αM SubunitPublished by Elsevier BV ,1998
- A structure prediction for the ligand‐binding region of the integrin β subunit: evidence for the presence of a von Willebrand factor A domainFEBS Letters, 1997
- Paired natural cysteine mutation mapping: Aid to constraining models of protein tertiary structureProtein Science, 1995
- KIM127, an Antibody that Promotes Adhesion, Maps to a Region of CD18 that Includes Cysteine-Rich RepeatsCell Adhesion and Communication, 1995
- The gene organisation of the human β2 integrin subunit (CD18)FEBS Letters, 1991
- Cloning of the $beta; subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene familyCell, 1987
- A human lymphocyte‐associated antigen involved in cell‐mediated lympholysisEuropean Journal of Immunology, 1983