THE USE OF MICE IN TESTS OF IMMUNITY AGAINST YELLOW FEVER
Open Access
- 1 October 1931
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 54 (4), 533-555
- https://doi.org/10.1084/jem.54.4.533
Abstract
1. A method of testing sera for protective power against yellow fever is described and designated as the intraperitoneal protection test in mice. 2. The test consists essentially of the inoculation of mice intra-peritoneally with yellow fever virus, fixed for mice, together with the serum to be tested, and the simultaneous injection of starch solution into the brain to localize the virus. If the serum lacks protective power the mice die of yellow fever encephalitis. 3. The test is highly sensitive. Consequently it is useful in epidemiological studies to determine whether individuals have ever had yellow fever and in tests to find whether vaccinated persons or animals have in reality been immunized. 4. When mice were given large intraperitoneal injections of yellow fever virus fixed for mice, the virus could be recovered from the blood for 4 days although encephalitis did not occur. If the brain was mildly injured at the time of the intraperitoneal injection, the symptoms of yellow fever encephalitis appeared 6 days later, but the virus was then absent from the blood. 5. Strains of white mice vary greatly in their susceptibility to yellow fever.Keywords
This publication has 4 references indexed in Scilit:
- SUSCEPTIBILITY OF WHITE MICE TO THE VIRUS OF YELLOW FEVERScience, 1930
- THE RELATIONSHIP OF YELLOW FEVER OF THE WESTERN HEMISPHERE TO THAT OF AFRICA AND TO LEPTOSPIRAL JAUNDICEThe Journal of Experimental Medicine, 1930
- INFECTIVITY OF BLOOD DURING THE COURSE OF EXPERIMENTAL YELLOW FEVERThe Journal of Experimental Medicine, 1929
- THE RELATION OF THE MENINGES AND CHOROID PLEXUS TO POLIOMYELITIC INFECTIONThe Journal of Experimental Medicine, 1917